6 research outputs found

    Robust estimation of bacterial cell count from optical density

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    Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

    31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016): part one

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    Coronal Heating as Determined by the Solar Flare Frequency Distribution Obtained by Aggregating Case Studies

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    Flare frequency distributions represent a key approach to addressing one of the largest problems in solar and stellar physics: determining the mechanism that counter-intuitively heats coronae to temperatures that are orders of magnitude hotter than the corresponding photospheres. It is widely accepted that the magnetic field is responsible for the heating, but there are two competing mechanisms that could explain it: nanoflares or Alfv\'en waves. To date, neither can be directly observed. Nanoflares are, by definition, extremely small, but their aggregate energy release could represent a substantial heating mechanism, presuming they are sufficiently abundant. One way to test this presumption is via the flare frequency distribution, which describes how often flares of various energies occur. If the slope of the power law fitting the flare frequency distribution is above a critical threshold, α=2\alpha=2 as established in prior literature, then there should be a sufficient abundance of nanoflares to explain coronal heating. We performed >>600 case studies of solar flares, made possible by an unprecedented number of data analysts via three semesters of an undergraduate physics laboratory course. This allowed us to include two crucial, but nontrivial, analysis methods: pre-flare baseline subtraction and computation of the flare energy, which requires determining flare start and stop times. We aggregated the results of these analyses into a statistical study to determine that α=1.63±0.03\alpha = 1.63 \pm 0.03. This is below the critical threshold, suggesting that Alfv\'en waves are an important driver of coronal heating.Comment: 1,002 authors, 14 pages, 4 figures, 3 tables, published by The Astrophysical Journal on 2023-05-09, volume 948, page 7

    Measurement of the W±ZW^{\pm}Z boson pair-production cross section in pppp collisions at s=13\sqrt{s}=13 TeV with the ATLAS Detector

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    The production of W±ZW^{\pm}Z events in proton--proton collisions at a centre-of-mass energy of 13 TeV is measured with the ATLAS detector at the LHC. The collected data correspond to an integrated luminosity of 3.2 fb1^{-1}. The W±ZW^{\pm}Z candidates are reconstructed using leptonic decays of the gauge bosons into electrons or muons. The measured inclusive cross section in the detector fiducial region for leptonic decay modes is σW±Zνfid.=63.2±3.2\sigma_{W^\pm Z \rightarrow \ell^{'} \nu \ell \ell}^{\textrm{fid.}} = 63.2 \pm 3.2 (stat.) ±2.6\pm 2.6 (sys.) ±1.5\pm 1.5 (lumi.) fb. In comparison, the next-to-leading-order Standard Model prediction is 53.42.8+3.653.4^{+3.6}_{-2.8} fb. The extrapolation of the measurement from the fiducial to the total phase space yields σW±Ztot.=50.6±2.6\sigma_{W^{\pm}Z}^{\textrm{tot.}} = 50.6 \pm 2.6 (stat.) ±2.0\pm 2.0 (sys.) ±0.9\pm 0.9 (th.) ±1.2\pm 1.2 (lumi.) pb, in agreement with a recent next-to-next-to-leading-order calculation of 48.21.0+1.148.2^{+1.1}_{-1.0} pb. The cross section as a function of jet multiplicity is also measured, together with the charge-dependent W+ZW^+Z and WZW^-Z cross sections and their ratio
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